Revision 1
March 19, 1998
by Radiation, Science, and Health, Inc.,
Edited by J. Muckerheide

1.5
Molecular & Cellular Biology

"To investigate the radioadaptive response of normal cells to low- dose radiation, we irradiated human embryonic (HE) cells and HeLa cells with low-dose X-rays and examined the changes in sensitivity to subsequent high-dose X-irradiation using the trypan bluedye-exclusion test. When HE cells were irradiated by 200 cGy, the growth ratio of the living cells 5 days after the irradiation decreased to 37% of that of the cells which received no X-irradiation. When the cells received a conditioning irradiation of 10-20 cGy 4 h before the irradiation of 200 cGy, the relative growth ratios increased significantly to

45-53%, and a peak was reached at a conditioning dose of 13 cGy to the cells. This conditioning effect was not observed in HeLa cells. When the HE cells were suspended in a Ca2+ ion-free medium or TPA added to the medium while receiving the conditioning irradiation of 13 cGy, the effect of the conditioning dose was not observed. This indicates that normal cells show an adaptive response to low-dose radiation and become more radioresistant. These results suggest that gap junctional intercellular communication may play a role in radioadaptive responses in human cells."